Determination of ketone bodies in blood by headspace gas chromatography-mass spectrometry.
نویسندگان
چکیده
A gas chromatography-mass spectrometry (GC-MS) method for determination of ketone bodies (β-hydroxybutyrate, acetone, and acetoacetate) in blood is presented. The method is based on enzymatic oxidation of D-β-hydroxybutyrate to acetoacetate, followed by decarboxylation to acetone, which was quantified by the use of headspace GC-MS using acetone-(13)C(3) as an internal standard. The developed method was found to have intra- and total interday relative standard deviations < 10% for acetone+acetoacetate levels (∼25 to 8300 μM) and D-β-hydroxybutyrate levels (∼30 to 16500 μM). Recovery values varied from 98 to 107%, demonstrating the suitability of the method for measuring ketone bodies over a wide concentration range. The method has been applied to cases in which ketoacidosis was suspected as the cause of death in diabetics or chronic alcoholics, as well as to cases in which another cause of death was identified.
منابع مشابه
Simultaneous Determination of Disinfection By-products in Water Samples from Advanced Membrane Treatments by Headspace Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry
A headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) method has been developed for the simultaneously determination of 20 disinfection by-products (DBPs) in water samples from reverse osmosis (RO) membranes. Selected compounds belong to different families including: trihalomethanes (THMs), halogenated acetonitriles (HANs), halogenated nitromethanes ...
متن کاملDetermination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.
In studies where D-(-)-3-hydroxy[4,4,4-2H3]butyrate is employed as isotopic tracer in vivo, we have described a selected ion monitoring, gas-liquid chromatography-mass spectrometry micromethod which measures [2H3] tracer enrichment in 3-hydroxybutyrate and acetoacetate from 300-microliters blood samples. For plasma samples in the physiologic range, intra- and interassay precisions for each keto...
متن کاملIdentification of volatile organic compounds of some Trichoderma species using static headspace gas chromatography-mass spectrometry
Fungi release wide spectrum of volatile organic compounds (VOCs) that belong to several chemical groups with different biochemical origins such as monoterpenes, sesquiterpenes, alcohols, aldehydes, aromatic compounds, esters, furans, ketones, sulfur and nitrogen compounds. Trichoderma species are the most studied fungal biocontrol agents and are successfully used as biofungicides and biofertili...
متن کاملSimultaneous Determination of 13 Priority Polycyclic Aromatic Hydrocarbons in Tehran’s Tap Water and Water for Injection Samples Using Dispersive Liquid-Liquid Micro Extraction Method and Gas Chromatography-Mass Spectrometry
Abstract Polycyclic aromatic hydrocarbons (PAHs) are classified as persistent and carcinogenic organic pollutants. PAHs contamination has been reported in water. Many of relevant regulatory bodies such as EU and EPA have regulated the limit levels for PAHs in drinking water. In this study, 13 priority polycyclic aromatic hydrocarbons (PAHs) were determined in tap water samples of Tehran and wat...
متن کاملDetermination of 1,1,1,2,3,3,3-heptafluoropropane (HFP) in blood by headspace gas chromatography-mass spectrometry.
The inert and certified medical gas 1,1,1,2,3,3,3-heptafluoropropane (HFP) is commonly used as a propellant in medical sprays. HFP is also of interest as tracer gas for lung function measurements. In this work a sensitive method for quantitative determination of HFP in blood was developed using headspace gas chromatography-mass spectrometry with N2 as internal standard. The limits of detection ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of analytical toxicology
دوره 34 9 شماره
صفحات -
تاریخ انتشار 2010